Scientific technology & expertise

Westmead Histology

The Westmead Histology Facility is a wellestablished open facility, equipped with modern state of the art equipment. Staff are available to provide training and services for researchers, students, affiliates and commercial users. Histology is used to understand the underlying microscopic details and cellular elements within tissue samples in both healthy and disease states.

Techniques, technologies & services

Westmead Histology has a range of equipment to undertake high quality research, encompassing tissue processing, embedding, and microtomy as well as histochemical staining.

Services

  • Tissue Processing/embedding
  • Paraffin Sectioning
  • Cryo Sectioning
  • Antigen Retrieval
  • HE and Special Stain
  • Tissue microarray (TMA)

Equipment

  • Tissue processor, Excelsior AS
  • Embedding Console, Histo Centre 3
  • Microtomes, Leica RM2125RT and RM2125RTS
  • Cryostats, Microtome HM505E and Cryostar Nx70
  • Cassette writer, Printmate AS
  • Slide writer, Slidemate AS
  • Automatic coverslipper, CTM 6
  • Automatic IHC stainer, IntelliPATH FLX
  • Decloaking chamber, NxGen

Histochemical Staining

  • Hematoxylin and Eosin Stain (H & E)
  • Periodic acid-Schiff Stain (PAS) for glycogen, basement membrane and fungi
  • Sirius Red Stain for fibrotic tissue
  • Gomori Trichrome Stain for collagen
  • Masson’s Trichrome Stain for collagen
  • Peris Stain for iron
  • Verhoeff’s Van Giesen Stain for elastic fibers
  • Oil Red O Stain for lipid (for frozen sections)

Iron deposition in liver as shown by Perl’s stain

Salivary gland showing positive glycogen staining surrounded by fatty tissue by PAS stain

Westmead Research Hub (WRH) Facilities

Westmead Histology is one of nine core facilities at Westmead precinct. WRH core facilities are available for all WRH researchers, external researchers and industry partners. Learn more about what our other facilities have to offer.

Contact us

For more information on Westmead Research Hub facilities , contact our team at:

westmead.histology@wimr.org.au

Location

The Histology Facility is located on Level 1 of the Westmead Institute for Medical Research